CoraLite® Plus 488-conjugated ATX3,ATXN3 Monoclonal antibody
ATX3,ATXN3 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, pig
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F7E10
验证数据展示
经过测试的应用
| Positive FC detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 67057-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67057 targets ATX3,ATXN3 in FC (Intra) applications and shows reactivity with Human, mouse, pig samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | Human, mouse, pig |
| Immunogen | ATX3,ATXN3 fusion protein Ag6627 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ataxin 3 |
| Synonyms | AT3, ataxin 3, ATX3, ATXN3, JOS, MJD, MJD1, SCA3 |
| Calculated Molecular Weight | 370 aa, 43 kDa |
| Observed Molecular Weight | 41-45 kDa |
| GenBank Accession Number | BC033711 |
| Gene Symbol | ATXN3 |
| Gene ID (NCBI) | 4287 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P54252 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 ATX3,ATXN3 antibody CL488-67057 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
