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acetylated Tubulin(Lys40) Antibody 12 Publications

Mouse Monoclonal| Catalog number: 66200-1-Ig |CloneNo.: 7E5H8

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浓度: 700μg/ml

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Related Primary Antibodies

Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

acetylated Tubulin(Lys40) Antibody IF MDCK cells 66200-1-Ig

Immunofluorescent analysis of MDCK cells using 66200-1-Ig(acetylated Tubulin(Lys40) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

acetylated Tubulin(Lys40) Antibody IF MDCK cells 66200-1-Ig

Immunofluorescent images of MDCK cells stained for IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated AffiniPure Goat anti-Mouse IgG (H+L) for 66200-1-Ig.

acetylated Tubulin(Lys40) Antibody WB HEK-293 cells 66200-1-Ig

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66200-1-Ig (acetylated Tubulin(Lys40) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

acetylated Tubulin(Lys40) Antibody IF MDCK cells 66200-1-Ig

Immunofluorescent analysis of MDCK cells using 66200-1-Ig(acetylated Tubulin(Lys40) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

acetylated Tubulin(Lys40) Antibody IF MDCK cells 66200-1-Ig

Immunofluorescent analysis of MDCK cells using 66200-1-Ig(acetylated Tubulin(Lys40) antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

acetylated Tubulin(Lys40) Antibody IF mouse brain tissue 66200-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 66200-1-Ig (acetylated Tubulin(Lys40) antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

acetylated Tubulin(Lys40) Antibody IHC human lung cancer tissue 66200-1-Ig

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66200-1-Ig( acetylated Tubulin(Lys40) antibody at dilution of 1:50 (under 10x lens)

acetylated Tubulin(Lys40) Antibody IHC human lung cancer tissue 66200-1-Ig

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66200-1-Ig( acetylated Tubulin(Lys40) antibody at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse, rat, dog, pig, Drosophila
Positive WB detected in:
HEK-293 cells, mouse ovary tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue, L02 cells
Positive IHC detected in:
human lung cancer tissue, human gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MDCK cells, mouse brain tissue
Recommended dilution:
WB : 1:1000-1:10000
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
tubulin, alpha 1a
Calculated molecular weight:
52 kDa
Observed molecular weight:
55 kDa
GenBank accession number:
NM_006009
Gene ID (NCBI):
7846
Gene symbol
TUBA1A
Synonyms
Ac tubulin, acetylated alpha tubulin, Acetylated tubulin, Acetylated tubulin (Lys40), acetylated-a-tubulin, acetyl-a-tubulin, Ac-tubulin, alpha tubulin, Alpha tubulin 3, tubulin

Background

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549)


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