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acetylated Tubulin(Lys40) Monoclonal antibody 36 Publications

Mouse Monoclonal| Catalog number: 66200-1-Ig |CloneNo.: 7E5H8

浓度: 700 μg/ml

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Applications

Tested Applications:
IF, IHC, WB, ELISA
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Cited Applications:
IF, WB


Images See all

WB analysis using 66200-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66200-1-Ig (acetylated Tubulin(Lys40) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IF Staining of MDCK using 66200-1-Ig

Immunofluorescent images of MDCK cells stained for IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated AffiniPure Goat anti-Mouse IgG (H+L) for 66200-1-Ig.

IHC staining of mouse ovary using 66200-1-Ig

Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66200-1-Ig (acetylated Tubulin(Lys40) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Species specificity:
human, mouse, rat, dog, pig, bovine, Drosophila, swine
Positive WB detected in:
rat testis tissue, NCCIT cells, Neuro-2a cells, L02 cells, pig cerebellum tissue, rat brain tissue, mouse ovary tissue, HEK-293 cells, mouse testis
Positive IHC detected in:
mouse ovary tissue, human gliomas tissue, rat brain tissue, mouse brain tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MDCK cells, mouse brain tissue
Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:1000-1:4000
IF : 1:50-1:500

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
tubulin, alpha 1a
Calculated molecular weight:
52 kDa
Observed molecular weight:
55 kDa
GenBank accession number:
NM_006009
Gene ID (NCBI):
7846
Gene symbol
TUBA1A
Synonyms
Ac tubulin, acetylated alpha tubulin, Acetylated tubulin, Acetylated tubulin (Lys40), acetylated-a-tubulin, acetyl-a-tubulin, Ac-tubulin, alpha tubulin, Alpha tubulin 3, tubulin

Background

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549)

Protocols

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