CoraLite® Plus 488-conjugated AlaRS Monoclonal antibody
AlaRS Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C8H3
验证数据展示
经过测试的应用
| Positive IF detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 67909-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67909 targets AlaRS in IF applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | AlaRS fusion protein Ag11151 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | alanyl-tRNA synthetase |
| Synonyms | AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS |
| Calculated Molecular Weight | 968 aa, 107 kDa |
| Observed Molecular Weight | 107 kDa |
| GenBank Accession Number | BC011451 |
| Gene Symbol | AARS |
| Gene ID (NCBI) | 16 |
| RRID | AB_2919560 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P49588 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AlaRS antibody CL488-67909 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |