CoraLite® Plus 488-conjugated Alpha 1 Antitrypsin Polyclonal antibody
Alpha 1 Antitrypsin Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SERPINA1/AAT Ready-To-Use IHC Kit. SERPINA1/AAT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16382-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-16382 targets Alpha 1 Antitrypsin in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) |
Tested Reactivity | human, mouse, rat |
Immunogen | Alpha 1 Antitrypsin fusion protein Ag9369 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 |
Synonyms | A1A, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT |
Calculated Molecular Weight | 418 aa, 47 kDa |
Observed Molecular Weight | 47 kDa |
GenBank Accession Number | BC015642 |
Gene Symbol | SERPINA1 |
Gene ID (NCBI) | 5265 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01009 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 Alpha 1 Antitrypsin antibody CL488-16382 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |