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CoraLite® Plus 647-conjugated Arginase-1 Polyclonal antibody

Arginase-1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-16001

Print datasheet

Synonyms

ARG1, Arginase 1, arginase, liver, Liver type arginase, Type I arginase

验证数据展示

  • FC experiment of HepG2 using CL647-16001

    FC experiment of HepG2 using CL647-16001

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Arginase-1 (CL647-16001) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16001-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-16001 targets Arginase-1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen Arginase-1 fusion protein Ag8595 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name arginase, liver
Synonyms ARG1, Arginase 1, arginase, liver, Liver type arginase, Type I arginase
Calculated Molecular Weight236aa,25 kDa; 322aa,35 kDa
Observed Molecular Weight 35-36 kDa
GenBank Accession NumberBC005321
Gene Symbol ARG1
Gene ID (NCBI) 383
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05089
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). ARG1 mainly expresses in neurons in a normal brain. The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Arginase-1 antibody CL647-16001Download protocol
Standard Protocols
Click here to view our Standard Protocols