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  • Featured Product
  • KD/KO Validated

BAX Monoclonal antibody, PBS Only

BAX Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,FC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB,IP,Indirect ELISA,IHC,FC

Conjugate

Unconjugated

CloneNo.

4G5E8

Cat No : 60267-1-PBS

Print datasheet

Synonyms

Apoptosis regulator BAX, BAX

验证数据展示

  • WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

    WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BAX antibody included
  • IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BAX antibody included
  • FC experiment of MCF-7 using 60267-1-Ig (same clone as 60267-1-PBS)

    FC experiment of MCF-7 using 60267-1-Ig (same clone as 60267-1-PBS)

    1X10^6 MCF-7 cells were stained with 0.2 ug Anti-Human BAX (60267-1-Ig, Clone:4G5E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

  • IP experiment of THP-1 using 60267-1-Ig (same clone as 60267-1-PBS)

    IP experiment of THP-1 using 60267-1-Ig (same clone as 60267-1-PBS)

    IP result of anti-BAX (IP:60267-1-Ig, 4ug; Detection:60267-1-Ig 1:6000) with THP-1 cells lysate 1520 ug. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy BAX Ready-To-Use IHC Kit. BAX primary antibody included.

产品信息

60267-1-PBS targets BAX in WB, IP, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,IP,Indirect ELISA,IHC,FC
Tested Reactivity human, mouse, rat
Immunogen BAX fusion protein Ag21068 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name BCL2-associated X protein
Synonyms Apoptosis regulator BAX, BAX
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC014175
Gene Symbol BAX
Gene ID (NCBI) 581
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07812
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).