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  • KD/KO Validated

BBS4 Polyclonal antibody

BBS4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12766-1-AP

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Synonyms

Bardet Biedl syndrome 4, BBS4



经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12766-1-AP targets BBS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen BBS4 fusion protein Ag3430 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Bardet-Biedl syndrome 4
Synonyms Bardet Biedl syndrome 4, BBS4
Calculated Molecular Weight 519 aa, 58 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC027624
Gene Symbol BBS4
Gene ID (NCBI) 585
RRIDAB_10596774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

实验方案

Product Specific Protocols
WB protocol for BBS4 antibody 12766-1-APDownload protocol
IHC protocol for BBS4 antibody 12766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.

Authors - Dragomir B Krastev
mouseWB,IF

PLoS Biol

Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.

Authors - Naila Haq
  • KO Validated
humanIF

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
mouseIF

Autophagy

The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.

Authors - Andreas Struchtrup
humanWB

EMBO Rep

Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.

Authors - Melis D Arslanhan
mouseWB

Elife

Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling.

Authors - Yan Xiong