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BCL9 Polyclonal antibody

BCL9 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22947-1-AP

Print datasheet

Synonyms

B cell CLL/lymphoma 9, B cell CLL/lymphoma 9 protein, B cell lymphoma 9 protein, BCL9, LGS, Protein legless homolog

验证数据展示

  • WB analysis using 22947-1-AP

    WB analysis using 22947-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22947-1-AP (BCL9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 22947-1-AP

    IF Staining of HeLa using 22947-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22947-1-AP (BCL9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 22947-1-AP

    IHC staining of human liver cancer using 22947-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22947-1-AP (BCL9 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 22947-1-AP

    IHC staining of human liver cancer using 22947-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22947-1-AP (BCL9 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells, HEK-293 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22947-1-AP targets BCL9 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen BCL9 fusion protein Ag19158 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell CLL/lymphoma 9
Synonyms B cell CLL/lymphoma 9, B cell CLL/lymphoma 9 protein, B cell lymphoma 9 protein, BCL9, LGS, Protein legless homolog
Calculated Molecular Weight 1426 aa, 149 kDa
Observed Molecular Weight 149 kDa
GenBank Accession NumberBC116451
Gene Symbol BCL9
Gene ID (NCBI) 607
RRIDAB_2879189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00512
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

实验方案

Product Specific Protocols
WB protocol for BCL9 antibody 22947-1-APDownload protocol
IHC protocol for BCL9 antibody 22947-1-APDownload protocol
IF protocol for BCL9 antibody 22947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bioorg Chem

Discovery of 2-(isoxazol-5-yl)phenyl 3,4-dihydroxybenzoate as a potential inhibitor for the Wnt/β-catenin pathway

Authors - Yongxi Dong
View Article
human,mouseWB,IHC

Reprod Biol Endocrinol

Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis.

Authors - Xi Wang
View Article
humanWB,IF,IP

J Biol Chem

USP9X-mediated deubiquitination of B cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.

Authors - Zesen Shang
View Article