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BOP1 Polyclonal antibody

BOP1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 28366-1-AP

Print datasheet

Synonyms

block of proliferation 1, BOP1, KIAA0124

验证数据展示

  • WB analysis using 28366-1-AP

    WB analysis using 28366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28366-1-AP (BOP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 28366-1-AP

    IF Staining of PC-3 using 28366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 28366-1-AP (BOP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 28366-1-AP

    IHC staining of human colon cancer using 28366-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 28366-1-AP

    IHC staining of human colon cancer using 28366-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inDU 145 cells, HeLa cells, NCI-H1299 cells
Positive IHC detected inhuman colon cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

28366-1-AP targets BOP1 in WB, IHC, IF applications and shows reactivity with Human, Mouse samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited Applications WB
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen BOP1 fusion protein Ag26669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name block of proliferation 1
Synonyms block of proliferation 1, BOP1, KIAA0124
Calculated Molecular Weight 84 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC013787
Gene Symbol BOP1
Gene ID (NCBI) 23246
RRIDAB_2881123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14137
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers(PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article(PMID: 30100995).

实验方案

Product Specific Protocols
WB protocol for BOP1 antibody 28366-1-APDownload protocol
IHC protocol for BOP1 antibody 28366-1-APDownload protocol
IF protocol for BOP1 antibody 28366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.

Authors - Miao Bai
View Article
humanWB

Front Oncol

Suppression of Ribosome Biogenesis by Targeting WD Repeat Domain 12 (WDR12) Inhibits Glioma Stem-Like Cell Growth.

Authors - Lanjuan Mi
View Article