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验证数据展示

  • WB analysis of HeLa using 20983-1-AP

    WB analysis of HeLa using 20983-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20983-1-AP (BRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

BRIP1(C-terminal) Polyclonal antibody

BRIP1(C-terminal) Polyclonal Antibody for WB, ELISA
Cat No. 20983-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

BACH1, BRIP1, BRIP1(C-terminal), FANCJ, OF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20983-1-AP targets BRIP1(C-terminal) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BRCA1 interacting protein C-terminal helicase 1
别名 BACH1, BRIP1, BRIP1(C-terminal), FANCJ, OF
计算分子量 141 kDa
观测分子量 140-150 kDa
GenBank蛋白编号NM_032043
基因名称 BACH1/BRIP1
Gene ID (NCBI) 83990
RRIDAB_2878782
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BX63
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRIP1, also named as BACH1 and FANCJ, belongs to the DEAD box helicase family and DEAH subfamily. It is a DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. It is involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Defects in BRIP1 are a cause of susceptibility to breast cancer (BC). Defects in BRIP1 are the cause of Fanconi anemia complementation group J (FANCJ). The antibody is specific to BRIP1.

实验方案

Product Specific Protocols
WB protocol for BRIP1(C-terminal) antibody 20983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols