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BTN3A3 Polyclonal antibody

BTN3A3 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15896-1-AP

Print datasheet

Synonyms

BTF3, BTN3A3

验证数据展示

  • WB analysis of Jurkat using 15896-1-AP

    WB analysis of Jurkat using 15896-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15896-1-AP (BTN3A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15896-1-AP

    IF Staining of HepG2 using 15896-1-AP

    Immunofluorescent analysis of HepG2 cells, using BTN3A3 antibody 15896-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of Jurkat using 15896-1-AP

    IP experiment of Jurkat using 15896-1-AP

    IP result of anti-BTN3A3 (IP:15896-1-AP, 4ug; Detection:15896-1-AP 1:500) with Jurkat cells lysate 4000ug.

经过测试的应用

Positive WB detected inJurkat cells, U-937 cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15896-1-AP targets BTN3A3 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IP, WB,ELISA
Tested Reactivity human
Immunogen BTN3A3 fusion protein Ag8711 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name butyrophilin, subfamily 3, member A3
Synonyms BTF3, BTN3A3
Calculated Molecular Weight 584 aa, 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC015815
Gene Symbol BTN3A3
Gene ID (NCBI) 10384
RRIDAB_2065784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00478
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTN3A3 antibody 15896-1-APDownload protocol
IF protocol for BTN3A3 antibody 15896-1-APDownload protocol
IP protocol for BTN3A3 antibody 15896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols