• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Beta Catenin Monoclonal antibody, PBS Only

Beta Catenin Monoclonal Antibody for WB,IP,IHC,IF,FC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

CloneNo.

1B8A1

Cat No : 66379-1-PBS

Print datasheet

Synonyms

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286, β-Catenin

验证数据展示

  • WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)

    WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 66379-1-Ig (same clone as 66379-1-PBS)

    IP experiment of mouse brain using 66379-1-Ig (same clone as 66379-1-PBS)

    IP result of anti-Beta Catenin (IP:66379-1-Ig, 4ug; Detection:66379-1-Ig 1:2000) with mouse brain tissue lysate 4400 ug. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    IF Staining of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    FC experiment of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human Beta Catenin (66379-1-Ig, Clone:1B8A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

产品信息

66379-1-PBS targets Beta Catenin in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB,IP,IHC,IF,FC,ELISA
Tested Reactivity human, mouse, rat, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286, β-Catenin
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberNM_001904
Gene Symbol CTNNB1
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35222
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)