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Beta Tubulin Monoclonal antibody, PBS Only

Beta Tubulin Monoclonal Antibody for WB,IP,IHC,IF,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, nematode, pig, zebrafish

Applications

WB,IP,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D4A4

Cat No : 66240-1-PBS

Print datasheet

Synonyms

beta tubulin, B-tubulin, MC1R, TUBB, Tubulin-beta

验证数据展示

  • WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

    WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

  • WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

    WB analysis using 66240-1-Ig (same clone as 66240-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66240-1-Ig (beta Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66240-1-Ig (beta Tubulin antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

    IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

    IHC staining of human brain using 66240-1-Ig (same clone as 66240-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66240-1-Ig (beta Tubulin antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IP experiment of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    IP experiment of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    IP result of anti-Beta Tubulin (IP:66240-1-Ig, 5ug; Detection:66240-1-Ig 1:1000) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    IF Staining of HeLa using 66240-1-Ig (same clone as 66240-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (66240-1-Ig, Clone: 1D4A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66240-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.

产品信息

66240-1-PBS targets Beta Tubulin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, nematode, pig, zebrafish samples.

Tested Applications WB,IP,IHC,IF,ELISA
Tested Reactivity human, mouse, rat, nematode, pig, zebrafish
Immunogen Beta Tubulin fusion protein Ag0117 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms beta tubulin, B-tubulin, MC1R, TUBB, Tubulin-beta
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13509
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.