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C1QBP Monoclonal antibody

C1QBP Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F9B1

Cat No : 68084-1-Ig

Print datasheet

Synonyms

C1QBP, gC1Q R, GC1q R protein, GC1QBP, gC1qR, Glycoprotein gC1qBP, HABP1, Hyaluronan binding protein 1, p32, p33, SF2p32

验证数据展示

  • WB analysis using 68084-1-Ig

    WB analysis using 68084-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68084-1-Ig (C1QBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of HeLa using 68084-1-Ig

    IF Staining of HeLa using 68084-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C1QBP antibody (68084-1-Ig, Clone: 1F9B1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human tonsillitis using 68084-1-Ig

    IHC staining of human tonsillitis using 68084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1QBP antibody included
  • IHC staining of human tonsillitis using 68084-1-Ig

    IHC staining of human tonsillitis using 68084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1QBP antibody included
  • FC experiment of HeLa using 68084-1-Ig

    FC experiment of HeLa using 68084-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human C1QBP (68084-1-Ig, Clone:1F9B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inLNCaP cells, mouse brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy C1QBP Ready-To-Use IHC Kit. C1QBP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68084-1-Ig targets C1QBP in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Mouse
Immunogen C1QBP fusion protein Ag19773 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name complement component 1, q subcomponent binding protein
Synonyms C1QBP, gC1Q R, GC1q R protein, GC1QBP, gC1qR, Glycoprotein gC1qBP, HABP1, Hyaluronan binding protein 1, p32, p33, SF2p32
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC013731
Gene Symbol C1QBP
Gene ID (NCBI) 708
RRIDAB_2918821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07021
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).

实验方案

Product Specific Protocols
WB protocol for C1QBP antibody 68084-1-IgDownload protocol
IHC protocol for C1QBP antibody 68084-1-IgDownload protocol
IF protocol for C1QBP antibody 68084-1-IgDownload protocol
FC protocol for C1QBP antibody 68084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols