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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated C3/C3b/C3c Polyclonal antibody

C3/C3b/C3c Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-21337

Print datasheet

Synonyms

ARMD9, ASP, C3, Complement C3, complement component 3, CPAMD1

验证数据展示

  • IF Staining of HeLa using CL488-21337

    IF Staining of HeLa using CL488-21337

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 C3/C3b/C3c antibody (CL488-21337) at dilution of 1:200.

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy C3 Ready-To-Use IHC Kit. C3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21337-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21337 targets C3/C3b/C3c in IF applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF/ICC
Tested Reactivity human, rat, mouse
Immunogen C3/C3b/C3c fusion protein Ag15537 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name complement component 3
Synonyms ARMD9, ASP, C3, Complement C3, complement component 3, CPAMD1
Calculated Molecular Weight 1663 aa, 187 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC150179
Gene Symbol C3
Gene ID (NCBI) 718
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01024
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The complement system is an important effector which bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg, and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C3/C3b/C3c antibody CL488-21337Download protocol
Standard Protocols
Click here to view our Standard Protocols