验证数据展示
经过测试的应用
| Positive WB detected in | human blood tissue, human plasma tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
11819-1-AP targets C4BPA in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | C4BPA fusion protein Ag2387 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | complement component 4 binding protein, alpha |
| 别名 | C4BP, C4BPA, Proline rich protein, PRP |
| 计算分子量 | 597 aa, 67 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC022312 |
| 基因名称 | C4BPA |
| Gene ID (NCBI) | 722 |
| RRID | AB_2877796 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04003 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP alpha chain (C4BPA).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C4BPA antibody 11819-1-AP | Download protocol |
| IHC protocol for C4BPA antibody 11819-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Hepatitis B virus X protein up-regulates C4b-binding protein α through activating transcription factor Sp1 in protection of hepatoma cells from complement attack. | ||
BMC Cancer The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer. | ||
World J Surg Oncol Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma |
