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MUC1/CA15-3 C-terminal Polyclonal antibody
MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
FC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, PC-3 cells, BxPC-3 cells, MCF-7 cells |
| Positive IHC detected in | human pancreas cancer tissue, human tonsillitis tissue, human breast cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy MUC1 Ready-To-Use IHC Kit. MUC1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23614-1-AP targets MUC1/CA15-3 C-terminal in WB, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB, FC, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | MUC1/CA15-3 C-terminal fusion protein Ag20366 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mucin 1, cell surface associated |
| Synonyms | CA15 3, CA153, CA15-3, Carcinoma associated mucin, CD227, EMA, Episialin, H23AG, KL-6, MAM6, MUC 1, MUC1, MUC1 beta, MUC1 CT, MUC1 NT, MUC1/CA15-3 C-terminal, Mucin 1, Peanut reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor associated mucin |
| Calculated Molecular Weight | 1264 aa, 123 kDa |
| Observed Molecular Weight | 23-28 kDa |
| GenBank Accession Number | BC120975 |
| Gene Symbol | MUC1 |
| Gene ID (NCBI) | 4582 |
| RRID | AB_2879304 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15941 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP | Download protocol |
| IHC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP | Download protocol |
| FC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Sens Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. | ||
Cell Cycle LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway. | ||
Pathol Res Pract MUC1 promotes cancer stemness and predicts poor prognosis in osteosarcoma
| ||
Cell Death Discov Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling. | ||
Cell Death Discov Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion. | ||
Front Oncol A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma |
