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验证数据展示

  • IHC staining of human brain using 27225-1-AP

    IHC staining of human brain using 27225-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27225-1-AP (CACNA1E Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 27225-1-AP

    IHC staining of human brain using 27225-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27225-1-AP (CACNA1E Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CACNA1E Polyclonal antibody

CACNA1E Polyclonal Antibody for IHC, ELISA
Cat No. 27225-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA and More (1)

别名

BII, Brain calcium channel II, CACH6, CACNA1E, CACNL1A6, Cav2.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27225-1-AP targets CACNA1E in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 CACNA1E fusion protein Ag25944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium channel, voltage-dependent, R type, alpha 1E subunit
别名 BII, Brain calcium channel II, CACH6, CACNA1E, CACNL1A6, Cav2.3
计算分子量 262 kDa
GenBank蛋白编号NM_000721
基因名称 CACNA1E
Gene ID (NCBI) 777
RRIDAB_2880808
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CACNA1E antibody 27225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Elife

β2-subunit alternative splicing stabilizes Cav2.3 Ca2+ channel activity during continuous midbrain dopamine neuron-like activity.

Authors - Anita Siller
View Article