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CALD1 Monoclonal antibody

CALD1 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat and More (1)

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E1C11

Cat No : 66693-1-Ig

Print datasheet

Synonyms

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

验证数据展示

  • WB analysis using 66693-1-Ig

    WB analysis using 66693-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66693-1-Ig

    WB analysis of HeLa using 66693-1-Ig

    WB result of CALD1 antibody (66693-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CALD1 transfected HeLa cells.

  • IHC staining of human colon cancer using 66693-1-Ig

    IHC staining of human colon cancer using 66693-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CALD1 antibody included
  • IHC staining of human colon cancer using 66693-1-Ig

    IHC staining of human colon cancer using 66693-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CALD1 antibody included
  • IF Staining of HepG2 using 66693-1-Ig

    IF Staining of HepG2 using 66693-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66693-1-Ig

    FC experiment of HeLa using 66693-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CALD1 (66693-1-Ig, Clone:1E1C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inNCI-H1299 cells, HeLa cells, HepG2 cells, PC-3 cells, SKOV-3 cells, A2780 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue, human hysteromyoma tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CALD1 Ready-To-Use IHC Kit. CALD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66693-1-Ig targets CALD1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Immunogen CALD1 fusion protein Ag13676 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name caldesmon 1
Synonyms CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22
Calculated Molecular Weight 538 aa, 63 kDa
Observed Molecular Weight 70 kDa~75 kDa
GenBank Accession NumberBC040354
Gene Symbol CALD1
Gene ID (NCBI) 800
RRIDAB_2882047
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05682
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CALD1 antibody 66693-1-IgDownload protocol
IHC protocol for CALD1 antibody 66693-1-IgDownload protocol
IF protocol for CALD1 antibody 66693-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Changes in the Expression of Smooth Muscle Cell-Related Genes in Human Dermal Sheath Cup Cells Associated with the Treatment Outcome of Autologous Cell-Based Therapy for Male and Female Pattern Hair Loss.

Authors - Yuzo Yoshida
View Article