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验证数据展示

  • WB analysis of NIH/3T3 using 66446-1-Ig

    WB analysis of NIH/3T3 using 66446-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66446-1-Ig

    IF Staining of HepG2 using 66446-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX3 antibody (66446-1-Ig, Clone: 1A5A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

CBX3 Monoclonal antibody

CBX3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66446-1-Ig

产品说明书

CloneNo. 1A5A3
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells, RAW 264.7 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CBX3 Ready-To-Use IHC Kit. CBX3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66446-1-Ig targets CBX3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 CBX3 fusion protein Ag19112 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 chromobox homolog 3 (HP1 gamma homolog, Drosophila)
别名 CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein
计算分子量 21 kDa
观测分子量 22 kDa
GenBank蛋白编号BC000954
基因名称 CBX3
Gene ID (NCBI) 11335
RRIDAB_2881815
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13185
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

实验方案

Product Specific Protocols
WB protocol for CBX3 antibody 66446-1-IgDownload protocol
IF protocol for CBX3 antibody 66446-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biochem Cell Biol

NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.

Authors - Dan Wang
View Article
humanWB

Adv Biol (Weinh)

CBX3 Downregulates HLTF to Activate PI3K/AKT Signaling Promoting Cholangiocarcinoma

Authors - Min Xie
View Article