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CCR2a-specific Polyclonal antibody

CCR2a-specific Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16153-1-AP

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Synonyms

C C chemokine receptor type 2, C C CKR 2, CC CKR 2, CCR 2, CCR2, CCR2a, CCR2a-specific, CCR2b, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP 1 R



经过测试的应用

Positive WB detected inJurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16153-1-AP targets CCR2a-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-C motif) receptor 2
Synonyms C C chemokine receptor type 2, C C CKR 2, CC CKR 2, CCR 2, CCR2, CCR2a, CCR2a-specific, CCR2b, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP 1 R
Calculated Molecular Weight 42 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberNM_000647
Gene Symbol CCR2
Gene ID (NCBI) 729230
RRIDAB_2262945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.

实验方案

Product Specific Protocols
WB protocol for CCR2a-specific antibody 16153-1-APDownload protocol
IHC protocol for CCR2a-specific antibody 16153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Lab Invest

Matrix metalloproteinase 11 (MMP11) in macrophages promotes the migration of HER2-positive breast cancer cells and monocyte recruitment through CCL2-CCR2 signaling.

Authors - Shin Ung Kang
humanWB

Oncol Rep

CCL2/CCR2 axis induces hepatocellular carcinoma invasion and epithelial-mesenchymal transition in vitro through activation of the Hedgehog pathway.

Authors - Huijie Zhuang
ratWB

J Ethnopharmacol

Identification of potential regulating effect of baicalin on NFκB/CCL2/CCR2 signaling pathway in rats with cerebral ischemia by antibody-based array and bioinformatics analysis

Authors - Tian Xu
mouseWB,IF

Mol Immunol

QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response.

Authors - Lele Deng
human,ratWB,IHC

J Physiol Biochem

Hypoxia-induced CCL2/CCR2 axis in adipose-derived stem cells (ADSCs) promotes angiogenesis by human dermal microvascular endothelial cells (HDMECs) in flap tissues

Authors - Bairong Fang
humanIHC

Clin Exp Rheumatol

Curcumin alleviates inflammation in Takayasu's arteritis by blocking CCL2 overexpression in adventitial fibroblasts.

Authors - Sifan Wu