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Anti-Mouse CD11c (N418)

CD11c Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

N418

Cat No : 65130-1-Ig

Print datasheet

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using 65130-1-Ig

    FC experiment of C57BL/6 mouse splenocytes using 65130-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) and 0.5 ug Anti-Mouse CD11c (65130-1-Ig, Clone:N418) and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100, or 0.5 ug Armenian Hamster IgG Isotype Control (PIP) and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100. Cells were not fixed.

经过测试的应用

Positive FC detected inC57BL/6 mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65130-1-Ig targets CD11c in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen Mouse spleen dendritic cells 种属同源性预测
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Full Name integrin alpha X
Synonyms AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418
GenBank Accession NumberBC167225
Gene Symbol Itgax
Gene ID (NCBI) 16411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9QXH4
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

实验方案

Product Specific Protocols
FC protocol for CD11c antibody 65130-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols