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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using APC-65145

    FC experiment of human peripheral blood lymphocytes using APC-65145

    100 ul human peripheral blood were surface stained with FITC Anti-Human CD3 (FITC-65151, Clone: UCHT1) and 5.00 ul APC Anti-Human CD19 (APC-65145, Clone: SJ25C1). Lymphocytes were gated for analysis. Cells were not fixed.

  • FC experiment of human peripheral blood lymphocytes using APC-65145

    FC experiment of human peripheral blood lymphocytes using APC-65145

    100 ul human peripheral blood were surface stained with FITC Anti-Human CD3 (FITC-65151, Clone: UCHT1) and APC Mouse IgG1 isotype control antibody. Lymphocytes were gated for analysis. Cells were not fixed.

APC Anti-Human CD19 (SJ25C1)

CD19 Monoclonal Antibody for FC
Cat No. APC-65145

Print datasheet

CloneNo. SJ25C1

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

Differentiation antigen CD19, CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size: 

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经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65145 targets CD19 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen NALM1 + NALM16 cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD19 molecule
Synonyms Differentiation antigen CD19, CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4
Calculated Molecular Weight 556 aa, 61 kDa
GenBank Accession NumberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
ENSEMBL Gene IDENSG00000177455
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP15391
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

实验方案

Product Specific Protocols
FC protocol for APC CD19 antibody APC-65145Download protocol
Standard Protocols
Click here to view our Standard Protocols