APC Anti-Mouse CD31 (390)
CD31 Monoclonal Antibody for FC, IF/ICC
Host / Isotype
Rat / IgG2a
Reactivity
Mouse and More (1)
Applications
FC, IF/ICC
Conjugate
APC Fluorescent Dye
CloneNo.
390
验证数据展示
经过测试的应用
| Positive IF detected in | bEnd.3 cells |
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
APC-65058 targets CD31 in IF, FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC, IF/ICC |
| Cited Applications | IF |
| Tested Reactivity | Mouse |
| Cited Reactivity | mouse |
| Immunogen | C3H/HeJ mouse hematopoietic progenitor cell line 32D 种属同源性预测 |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | platelet/endothelial cell adhesion molecule 1 |
| Synonyms | C85791, Cd31, Pecam, PECAM 1, Pecam1 |
| GenBank Accession Number | BC008519 |
| Gene Symbol | Pecam1 |
| Gene ID (NCBI) | 18613 |
| RRID | AB_2882960 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q08481 |
| Storage Buffer | PBS with 0.1% sodium azide and a stabilizer. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
背景介绍
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for APC CD31 antibody APC-65058 | Download protocol |
| FC protocol for APC CD31 antibody APC-65058 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomaterials Synergetic regulation of kupffer cells, extracellular matrix and hepatic stellate cells with versatile CXCR4-inhibiting nanocomplex for magnified therapy in liver fibrosis. |
