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CD33 Monoclonal antibody

CD33 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2E5

Cat No : 67135-1-Ig

Print datasheet

Synonyms

CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3

验证数据展示

  • WB analysis of THP-1 using 67135-1-Ig

    WB analysis of THP-1 using 67135-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67135-1-Ig (CD33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 67135-1-Ig

    IHC staining of human spleen using 67135-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 67135-1-Ig

    IHC staining of human spleen using 67135-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 67135-1-Ig

    IF Staining of THP-1 using 67135-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD33 antibody (67135-1-Ig, Clone: 1B2E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67135-1-Ig targets CD33 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen CD33 fusion protein Ag11402 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD33 molecule
Synonyms CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3
Calculated Molecular Weight 364 aa, 40 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC028152
Gene Symbol CD33
Gene ID (NCBI) 945
RRIDAB_2882434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20138
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

实验方案

Product Specific Protocols
WB protocol for CD33 antibody 67135-1-IgDownload protocol
IHC protocol for CD33 antibody 67135-1-IgDownload protocol
IF protocol for CD33 antibody 67135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols