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CD45 Polyclonal antibody

CD45 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29914-1-AP

Print datasheet

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

验证数据展示

  • WB analysis using 29914-1-AP

    WB analysis using 29914-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29914-1-AP (CD45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 29914-1-AP

    IHC staining of human tonsillitis using 29914-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29914-1-AP (CD45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD45 antibody included
  • IHC staining of human tonsillitis using 29914-1-AP

    IHC staining of human tonsillitis using 29914-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29914-1-AP (CD45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD45 antibody included

经过测试的应用

Positive WB detected inJurkat cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29914-1-AP targets CD45 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen CD45 fusion protein Ag31286 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200
Calculated Molecular Weight 1304 aa, 147 kDa
Observed Molecular Weight180-250 kDa
GenBank Accession NumberBC014239
Gene Symbol PTPRC
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08575
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single-chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

实验方案

Product Specific Protocols
WB protocol for CD45 antibody 29914-1-APDownload protocol
IHC protocol for CD45 antibody 29914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols