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CoraLite®555-conjugated CD45 Recombinant antibody

CD45 Recombinant Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse

Applications

FC (Intra), IF-P

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

6O19

Cat No : CL555-80297

Print datasheet

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

验证数据展示

  • IF Staining of mouse spleen using CL555-80297

    IF Staining of mouse spleen using CL555-80297

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®555 CD45 antibody (CL555-80297, Clone: 6O19 ) at dilution of 1:200.

  • IF Staining of mouse spleen using CL555-80297

    IF Staining of mouse spleen using CL555-80297

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®555 CD45 antibody (CL555-80297, Clone: 6O19 ) at dilution of 1:200.

  • FC experiment of Raji using CL555-80297

    FC experiment of Raji using CL555-80297

    1X10^6 Raji cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CD45 (CL555-80297, Clone:6O19) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inmouse spleen tissue
Positive FC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80297-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-80297 targets CD45 in IF, FC (Intra) applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity Human, Rat, Mouse
Immunogen CD45 fusion protein Ag13712 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200
Calculated Molecular Weight 1304 aa, 147 kDa
Observed Molecular Weight220-240 kDa
GenBank Accession NumberBC014239
Gene Symbol PTPRC
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08575
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

实验方案

Product Specific Protocols
IF protocol for CL555 CD45 antibody CL555-80297Download protocol
FC protocol for CL555 CD45 antibody CL555-80297Download protocol
Standard Protocols
Click here to view our Standard Protocols