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CD47 Polyclonal antibody

CD47 Polyclonal Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

Cat No : 29555-1-AP

Print datasheet

Synonyms

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6

验证数据展示

  • FC experiment of HUVEC using 29555-1-AP

    FC experiment of HUVEC using 29555-1-AP

    1X10^6 HUVEC cells were surface stained with 0.2 ug Anti-Human CD47 (29555-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were not fixed .

经过测试的应用

Positive FC detected inHUVEC cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29555-1-AP targets CD47 in FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, ELISA
Tested Reactivity Human
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD47 molecule
Synonyms CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6
Calculated Molecular Weight 323 aa, 35 kDa
GenBank Accession NumberBC010016
Gene Symbol CD47
Gene ID (NCBI) 961
RRIDAB_2918325
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08722
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

实验方案

Product Specific Protocols
FC protocol for CD47 antibody 29555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols