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CoraLite®594-conjugated CDC25B Monoclonal antibody

CDC25B Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H3A4

Cat No : CL594-67145

Print datasheet

Synonyms

CDC25B, CDC25HU2, M phase inducer phosphatase 2

验证数据展示

  • IF Staining of Jurkat using CL594-67145

    IF Staining of Jurkat using CL594-67145

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 CDC25B antibody (CL594-67145, Clone: 1H3A4 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL594-67145

    FC experiment of Jurkat using CL594-67145

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CDC25B (CL594-67145, Clone:1H3A4) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CDC25B Ready-To-Use IHC Kit. CDC25B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67145-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67145 targets CDC25B in IF, FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, rat
Immunogen CDC25B fusion protein Ag27193 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cell division cycle 25 homolog B (S. pombe)
Synonyms CDC25B, CDC25HU2, M phase inducer phosphatase 2
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC006395
Gene Symbol CDC25B
Gene ID (NCBI) 994
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30305
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

实验方案

Product Specific Protocols
IF protocol for CL594 CDC25B antibody CL594-67145Download protocol
FC protocol for CL594 CDC25B antibody CL594-67145Download protocol
Standard Protocols
Click here to view our Standard Protocols