验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, COS-7 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells, A431 cells, MCF-7 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10218-1-AP targets CDC37 in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, RIP |
| 经测试反应性 | human, mouse, monkey |
| 文献引用反应性 | human, mouse |
| 免疫原 | CDC37 fusion protein Ag0267 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cell division cycle 37 homolog (S. cerevisiae) |
| 别名 | p50Cdc37, Hsp90 co-chaperone Cdc37, N-terminally processed, Hsp90 co-chaperone Cdc37, Hsp90 co chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit |
| 计算分子量 | 44 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC000083 |
| 基因名称 | CDC37 |
| Gene ID (NCBI) | 11140 |
| RRID | AB_2077735 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16543 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cdc (cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC37 antibody 10218-1-AP | Download protocol |
| IHC protocol for CDC37 antibody 10218-1-AP | Download protocol |
| IF protocol for CDC37 antibody 10218-1-AP | Download protocol |
| IP protocol for CDC37 antibody 10218-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
Comput Biol Med Identifying mitophagy-related genes as prognostic biomarkers and therapeutic targets of gastric carcinoma by integrated analysis of single-cell and bulk-RNA sequencing data | ||
Oncogene Non-canonical roles of PFKFB3 in regulation of cell cycle through binding to CDK4. | ||
Int J Mol Sci (20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells. | ||
Pharmacol Res Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect. | ||
Inflammation Inhibition of Cdc37 Ameliorates Arthritis in Collagen-Induced Arthritis Rats by Inhibiting Synoviocyte Proliferation and Migration Through the ERK Pathway
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