验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, mouse small intestine tissue |
| Positive IHC detected in | human colon cancer tissue, human colon tissue, human stomach cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CDH17 Ready-To-Use IHC Kit. CDH17 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24339-1-AP targets Cadherin-17 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Cadherin-17 fusion protein Ag19488 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cadherin 17, LI cadherin (liver-intestine) |
| 别名 | CDH17, Intestinal peptide-associated transporter HPT-1, HPT1, HPT 1, CDH16 |
| 计算分子量 | 832 aa, 92 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC113464 |
| 基因名称 | Cadherin-17 |
| Gene ID (NCBI) | 1015 |
| RRID | AB_2728752 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12864 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cadherin-17 antibody 24339-1-AP | Download protocol |
| IHC protocol for Cadherin-17 antibody 24339-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties. | ||
Jpn J Clin Oncol Establishing metastatic patient-derived xenograft model for colorectal cancer. | ||
J Biol Chem Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis | ||
Int J Mol Sci A Regulator Role for the ATP-Binding Cassette Subfamily C Member 6 Transporter in HepG2 Cells: Effect on the Dynamics of Cell-Cell and Cell-Matrix Interactions | ||
J Exp Clin Cancer Res A complex of cadherin 17 with desmocollin 1 and p120-catenin regulates colorectal cancer migration and invasion according to the cell phenotype
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