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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CDK5 Polyclonal antibody

CDK5 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10430

Print datasheet

Synonyms

CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit

验证数据展示

  • IF Staining of SH-SY5Y using CL488-10430

    IF Staining of SH-SY5Y using CL488-10430

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 CDK5 antibody (CL488-10430) at dilution of 1:200.

经过测试的应用

Positive IF detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy CDK5 Ready-To-Use IHC Kit. CDK5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10430-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10430 targets CDK5 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen CDK5 fusion protein Ag0686 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase 5
Synonyms CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit
Calculated Molecular Weight 31 kDa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC005115
Gene Symbol CDK5
Gene ID (NCBI) 1020
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00535
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CDK5 antibody CL488-10430Download protocol
Standard Protocols
Click here to view our Standard Protocols