验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, mouse embryo tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CDK7 Ready-To-Use IHC Kit. CDK7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
27027-1-AP targets CDK7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | CDK7 fusion protein Ag25726 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin-dependent kinase 7 |
| 别名 | CDKN7, CDK-activating kinase 1, CDK activating kinase, CAK1, CAK |
| 计算分子量 | 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC005298 |
| 基因名称 | CDK7 |
| Gene ID (NCBI) | 1022 |
| RRID | AB_2880728 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P50613 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK7 antibody 27027-1-AP | Download protocol |
| IHC protocol for CDK7 antibody 27027-1-AP | Download protocol |
| IF protocol for CDK7 antibody 27027-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Nanomaterial-Facilitated Cyclin-Dependent Kinase 7 Inhibition Suppresses Gallbladder Cancer Progression via Targeting Transcriptional Addiction. | ||
Front Oncol Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach | ||
Cell Signal TAGLN2 targeted control of ARPC5-mediated activation of the MEK/ERK signaling pathway influences the proliferation, invasion, and metastasis of pancreatic cancer cells | ||
J Immunother Cancer Adenovirus vaccine targeting kinases induces potent antitumor immunity in solid tumors | ||
Nat Commun CDK9 recruits HUWE1 to degrade RARα and offers therapeutic opportunities for cutaneous T-cell lymphoma |
