CEP135 Polyclonal antibody
CEP135 Polyclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, Canine and More (1)
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, K-562 cells, U2OS cells, U-87 MG cells |
Positive IF detected in | MDCK cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:3000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 6 publications below |
产品信息
24428-1-AP targets CEP135 in WB, IF, ELISA applications and shows reactivity with human, Canine samples.
Tested Applications | IF/ICC, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human, Canine |
Cited Reactivity | human, mouse |
Immunogen | CEP135 fusion protein Ag19122 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | centrosomal protein 135kDa |
Synonyms | centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635 |
Calculated Molecular Weight | 1140 aa, 134 kDa |
Observed Molecular Weight | 135 kDa |
GenBank Accession Number | BC136535 |
Gene Symbol | CEP135 |
Gene ID (NCBI) | 9662 |
RRID | AB_2879543 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q66GS9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CEP135 antibody 24428-1-AP | Download protocol |
IF protocol for CEP135 antibody 24428-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Curr Biol SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells. | ||
EMBO Rep ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. | ||
Elife WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity. | ||
Cells Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells. | ||
Mol Biol Cell The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis. | ||
Gene A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF). |