验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells, HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14498-1-AP targets CEP250/CNAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | CEP250/CNAP1 fusion protein Ag5925 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | centrosomal protein 250kDa |
| Synonyms | Cep250, CEP250,C-NAP1, Centrosome-associated protein CEP250, centrosomal protein 250kDa, Centrosomal protein 2 |
| Calculated Molecular Weight | 281 kDa |
| Observed Molecular Weight | 250 kDa |
| GenBank Accession Number | BC001433 |
| Gene Symbol | CEP250/CNAP1 |
| Gene ID (NCBI) | 11190 |
| RRID | AB_2076918 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BV73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP250/CNAP1 antibody 14498-1-AP | Download protocol |
| IHC protocol for CEP250/CNAP1 antibody 14498-1-AP | Download protocol |
| IF protocol for CEP250/CNAP1 antibody 14498-1-AP | Download protocol |
| IP protocol for CEP250/CNAP1 antibody 14498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. | ||
Nat Commun Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis. | ||
PLoS Biol Stable centrosomal roots disentangle to allow interphase centriole independence. | ||
Proc Natl Acad Sci U S A Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins. | ||
J Cell Biol Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals. |
