CHOP; GADD153 Recombinant antibody
CHOP; GADD153 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G11
验证数据展示
经过测试的应用
Positive WB detected in | Tunicamycin treated HeLa cells, Tunicamycin treated Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy DDIT3 Ready-To-Use IHC Kit. DDIT3 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81462-1-RR targets CHOP; GADD153 in WB, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, ELISA |
Tested Reactivity | Human |
Immunogen | CHOP; GADD153 fusion protein Ag7354 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | DNA-damage-inducible transcript 3 |
Synonyms | C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153 |
Calculated Molecular Weight | 19 kDa |
Observed Molecular Weight | 30 kDa |
GenBank Accession Number | BC003637 |
Gene Symbol | DDIT3 |
Gene ID (NCBI) | 1649 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P35638 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).
实验方案
Product Specific Protocols | |
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WB protocol for CHOP; GADD153 antibody 81462-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |