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CoraLite® Plus 647-conjugated CITED1 Polyclonal antibody

CITED1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-26999

Print datasheet

Synonyms

CITED1, Melanocyte specific protein 1, MSG1

验证数据展示

  • FC experiment of HEK-293 using CL647-26999

    FC experiment of HEK-293 using CL647-26999

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CITED1 (CL647-26999) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CITED1 Ready-To-Use IHC Kit. CITED1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26999-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL647-26999 is CITED1 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra)
Tested Reactivity human, rat, mouse
Immunogen CITED1 fusion protein Ag24643 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
Synonyms CITED1, Melanocyte specific protein 1, MSG1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC004240
Gene Symbol CITED1
Gene ID (NCBI) 4435
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99966
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. CITED1 has 2 isoforms with the molecular mass of 20 and 23 kDa. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CITED1 antibody CL647-26999Download protocol
Standard Protocols
Click here to view our Standard Protocols