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  • Featured Product
  • KD/KO Validated

CNBP Monoclonal antibody

CNBP Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1D12

Cat No : 67109-1-Ig

Print datasheet

Synonyms

CNBP, CNBP1, DM2, FLJ11631, PROMM, RNF163, ZCCHC22, Zinc finger protein 9, ZNF9

验证数据展示

  • WB analysis using 67109-1-Ig

    WB analysis using 67109-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IP experiment of HeLa using 67109-1-Ig

    IP experiment of HeLa using 67109-1-Ig

    IP result of anti-CNBP (IP:67109-1-Ig, 5ug; Detection:67109-1-Ig 1:500) with HeLa cells lysate 1600 ug.

  • WB analysis using 67109-1-Ig

    WB analysis using 67109-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67109-1-Ig (CNBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, A549 cells, NCI-H1299 cells, Caco-2 cells, HT-29 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HepG2 cells, K-562 cells, PC-12 cells, Neuro-2a cells
Positive IP detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67109-1-Ig targets CNBP in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen CNBP fusion protein Ag7114 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CCHC-type zinc finger, nucleic acid binding protein
Synonyms CNBP, CNBP1, DM2, FLJ11631, PROMM, RNF163, ZCCHC22, Zinc finger protein 9, ZNF9
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19-25 kDa
GenBank Accession NumberBC000288
Gene Symbol CNBP
Gene ID (NCBI) 7555
RRIDAB_2882413
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNBP antibody 67109-1-IgDownload protocol
IP protocol for CNBP antibody 67109-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP,WB

Nat Microbiol

The SARS-CoV-2 RNA-protein interactome in infected human cells.

Authors - Nora Schmidt
  • KO Validated
View Article