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  • Featured Product
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Calponin Polyclonal antibody

Calponin Polyclonal Antibody for WB, IP, IF/ICC,IF-P, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF/ICC,IF-P, FC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 13938-1-AP

Print datasheet

Synonyms

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

验证数据展示

  • WB analysis using 13938-1-AP

    WB analysis using 13938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13938-1-AP (Calponin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse heart using 13938-1-AP

    IF Staining of mouse heart using 13938-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 13938-1-AP (Calponin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human heart using 13938-1-AP

    IHC staining of human heart using 13938-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 13938-1-AP

    IHC staining of human heart using 13938-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13938-1-AP (CNN1 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis of mouse heart using 13938-1-AP

    WB analysis of mouse heart using 13938-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13938-1-AP (CNN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 13938-1-AP

    IP experiment of mouse lung using 13938-1-AP

    IP result of anti-Calponin (IP:13938-1-AP, 4ug; Detection:13938-1-AP 1:1000) with mouse lung tissue lysate 2800ug.

  • IHC staining of human appendicitis using 13938-1-AP

    IHC staining of human appendicitis using 13938-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 13938-1-AP (Calponin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of C2C12 using 13938-1-AP

    IF Staining of C2C12 using 13938-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Calponin antibody (13938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of C2C12 using 13938-1-AP

    FC experiment of C2C12 using 13938-1-AP

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Calponin (13938-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse colon tissue, mouse heart tissue, mouse lung tissue, mouse uterus tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman appendicitis tissue, human ovary tumor tissue, human hysteromyoma tissue, human breast cancer tissue, human bladder tissue, human skin tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, mouse heart tissue
Positive FC detected inC2C12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13938-1-AP targets Calponin in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF/ICC,IF-P, FC, IHC, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen Calponin fusion protein Ag4989 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calponin 1, basic, smooth muscle
Synonyms Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC022015
Gene Symbol CNN1
Gene ID (NCBI) 1264
RRIDAB_2082010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51911
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the full-length of human CNN1 may cross-react with CNN2 and CNN3.

实验方案

Product Specific Protocols
WB protocol for Calponin antibody 13938-1-APDownload protocol
IHC protocol for Calponin antibody 13938-1-APDownload protocol
IF protocol for Calponin antibody 13938-1-APDownload protocol
IP protocol for Calponin antibody 13938-1-APDownload protocol
FC protocol for Calponin antibody 13938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Clin Invest

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Authors - Xue Gong
View Article
ratIF

Acta Biomater

Hybrid electrospun rapamycin-loaded small-diameter decellularized vascular grafts effectively inhibit intimal hyperplasia.

Authors - Yang Yang
View Article
mouseWB

Theranostics

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Authors - Gao-Shan Yang
View Article
ratWB

Phytomedicine

Betulinaldehyde inhibits vascular remodeling by regulating the microenvironment through the PLCγ1/Ca2+/MMP9 pathway

Authors - Yangxia Fu
View Article
mouseWB

Arterioscler Thromb Vasc Biol

CRISPR-Cas9 genome editing of a single regulatory element nearly abolishes target gene expression in mice--brief report.

Authors - Yu Han
View Article
humanWB

Genomics

Identification of transfer RNA-derived fragments and their potential roles in aortic dissection.

Authors - Xiuxiu Fu
View Article