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  • Featured Product
  • KD/KO Validated

CNOT1 Monoclonal antibody, PBS Only

CNOT1 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A10A11

Cat No : 66507-1-PBS

Print datasheet

Synonyms

AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H

验证数据展示

  • WB analysis of HeLa using 66507-1-Ig (same clone as 66507-1-PBS)

    WB analysis of HeLa using 66507-1-Ig (same clone as 66507-1-PBS)

    WB result of CNOT1 antibody (66507-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT1 transfected HeLa cells. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

    IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CNOT1 antibody included
  • IF Staining of HepG2 using 66507-1-Ig (same clone as 66507-1-PBS)

    IF Staining of HepG2 using 66507-1-Ig (same clone as 66507-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66507-1-Ig (CNOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CNOT1 Ready-To-Use IHC Kit. CNOT1 primary antibody included.

产品信息

66507-1-PBS targets CNOT1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB,IHC,IF,ELISA
Tested Reactivity Human, mouse, rat, pig
Immunogen CNOT1 fusion protein Ag5623 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CCR4-NOT transcription complex, subunit 1
Synonyms AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H
Calculated Molecular Weight 267 kDa
Observed Molecular Weight 240-250 kDa
GenBank Accession NumberBC040523
Gene Symbol CNOT1
Gene ID (NCBI) 23019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDA5YKK6
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.