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验证数据展示

  • WB analysis using 66729-1-Ig

    WB analysis using 66729-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 66729-1-Ig

    WB analysis of HepG2 using 66729-1-Ig

    WB result of CNPase antibody (66729-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPase transfected HepG2 cells.

  • IF Staining of mouse brain using 66729-1-Ig

    IF Staining of mouse brain using 66729-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HepG2 using 66729-1-Ig

    FC experiment of HepG2 using 66729-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CNPase (66729-1-Ig, Clone:1B9G9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CNPase Monoclonal antibody

CNPase Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 66729-1-Ig

Print datasheet

CloneNo. 1B9G9
Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, FC (Intra), ELISA and More (1)

Synonyms

CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase, 1B9G9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inpig cerebellum tissue, U-251 cells, human brain tissue, HepG2 cells, rat brain tissue, rat cerebellum tissue
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66729-1-Ig targets CNPase in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse, rat
Immunogen CNPase fusion protein Ag4227 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 2',3'-cyclic nucleotide 3' phosphodiesterase
Synonyms CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase, 1B9G9
Calculated Molecular Weight 421 aa, 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC028040
Gene Symbol CNPase
Gene ID (NCBI) 1267
RRIDAB_2882079
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.

实验方案

Product Specific Protocols
WB protocol for CNPase antibody 66729-1-IgDownload protocol
IF protocol for CNPase antibody 66729-1-IgDownload protocol
FC protocol for CNPase antibody 66729-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF,WB

CNS Neurosci Ther

EAAT3 impedes oligodendrocyte remyelination in chronic cerebral hypoperfusion-induced white matter injury

Authors - Yingmei Zhang
View Article
ratWB

J Ethnopharmacol

Shenzhiling oral liquid protects the myelin sheath against Alzheimer's disease through the PI3K/Akt-mTOR pathway.

Authors - Mingcui Zheng
View Article