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CoraLite®594-conjugated COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G2B9

Cat No : CL594-66351

Print datasheet

Synonyms

COX 2, COX2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

验证数据展示

  • IF Staining of mouse lung using CL594-66351

    IF Staining of mouse lung using CL594-66351

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using CoraLite®594 COX2/ Cyclooxygenase 2/ PTGS2 antibody (CL594-66351, Clone: 3G2B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF detected inmouse lung tissue
For other applications, we recommend the unconjugated version of this antibody, 66351-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66351 targets COX2/ Cyclooxygenase 2/ PTGS2 in IF applications and shows reactivity with human samples.

Tested Applications IF-P
Tested Reactivity human
Immunogen COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Synonyms COX 2, COX2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2
Calculated Molecular Weight 604 aa, 68 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC013734
Gene Symbol PTGS2
Gene ID (NCBI) 5743
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35354
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).

实验方案

Product Specific Protocols
IF protocol for CL594 COX2/ Cyclooxygenase 2/ PTGS2 antibody CL594-66351Download protocol
Standard Protocols
Click here to view our Standard Protocols