验证数据展示

WB analysis using 12208-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12208-1-AP (CREB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
ChIP experiment of HEK-293 using 12208-1-AP
Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 µg of cross-linked chromatin, 5 µg of CREB1 Antibody (12208-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.
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Chromatin was prepared from HEK-293 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 µg of cross-linked chromatin, 5 µg of CREB1 Antibody (12208-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.
WB analysis of HEK-293 using 12208-1-AP
WB result of CREB1 antibody (12208-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB1 transfected HEK-293 cells.
IP experiment of HEK-293 using 12208-1-AP
IP result of anti-CREB1 (IP:12208-1-AP, 4ug; Detection:12208-1-AP 1:2000) with HEK-293 cells lysate 1360 ug.
IHC staining of mouse brain using 12208-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12208-1-AP (CREB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CREB1 antibody included
FC experiment of HEK-293 using 12208-1-AP
1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human CREB1 (12208-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IF Staining of HeLa using 12208-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREB1 antibody (12208-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Featured Product
KD/KO验证

CREB1 Polyclonal antibody

CREB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Cat No. 12208-1-AP

产品说明书

引用文献(149)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey and More (3)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA and More (4)

别名

cAMP-responsive element-binding protein 1, CREB, CREB 1, CREB-1, Cyclic AMP-responsive element-binding protein 1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (149)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, rat brain tissue, multi-cells/tissue, Jurkat cells, COS-7 cells, mouse brain tissue, mouse lung tissue, HeLa cells, PC-3 cells, HL-60 cells, A431 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells, HepG2 cells, PC-12 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	mouse brain tissue, human cervical cancer tissue, human prostate cancer tissue, human thyroid tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HEK-293 cells
Positive FC (Intra) detected in	HEK-293 cells
Positive ChIP detected in	HEK-293 cells

Planning an IHC experiment? We recommend our IHCeasy CREB1 Ready-To-Use IHC Kit. CREB1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Chromatin immunoprecipitation (ChIP)	CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 7 publications below
WB	See 139 publications below
IHC	See 5 publications below
IF	See 5 publications below
IP	See 2 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below
ChIP	See 7 publications below
RIP	See 1 publications below

产品信息

12208-1-AP targets CREB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, chIP, RIP, ELISA
经测试反应性	human, mouse, rat, monkey
文献引用反应性	human, mouse, rat, chicken, goat, sea cucumbers
免疫原	CREB1 fusion protein Ag2852 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cAMP responsive element binding protein 1
别名	cAMP-responsive element-binding protein 1, CREB, CREB 1, CREB-1, Cyclic AMP-responsive element-binding protein 1
计算分子量	341 aa, 35 kDa
观测分子量	43-46 kDa
GenBank蛋白编号	BC010636
基因名称	CREB1
Gene ID (NCBI)	1385
RRID	AB_2245417
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P16220
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).

实验方案

Product Specific Protocols
WB protocol for CREB1 antibody 12208-1-AP	Download protocol
IHC protocol for CREB1 antibody 12208-1-AP	Download protocol
IF protocol for CREB1 antibody 12208-1-AP	Download protocol
IP protocol for CREB1 antibody 12208-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human,mouse	WB	Cell Metab Lactate modulates iron metabolism by binding soluble adenylyl cyclase Authors - Wei Liu View Article
human	WB	Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress. Authors - Qidong Li KD Validated View Article
mouse	WB	Mol Cell LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation Authors - Shelby E Compton View Article
human	WB	Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA. Authors - Bing Yao View Article
human,mouse	WB	Environ Int Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms Authors - Chaoyang Liu View Article
mouse	WB	Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke Authors - Haiyun Guo View Article

验证数据展示

WB analysis using 12208-1-AP

ChIP experiment of HEK-293 using 12208-1-AP

WB analysis of HEK-293 using 12208-1-AP

IP experiment of HEK-293 using 12208-1-AP

IHC staining of mouse brain using 12208-1-AP

FC experiment of HEK-293 using 12208-1-AP

IF Staining of HeLa using 12208-1-AP

CREB1 Polyclonal antibody

CREB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Lactate modulates iron metabolism by binding soluble adenylyl cyclase

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke