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CoraLite® Plus 488-conjugated CREB3L1,OASIS Monoclonal antibody

CREB3L1,OASIS Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B5B4

Cat No : CL488-67617

Print datasheet

Synonyms

CREB3L1, OASIS

验证数据展示

  • FC experiment of HepG2 using CL488-67617

    FC experiment of HepG2 using CL488-67617

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CREB3L1,OASIS (CL488-67617, Clone:2B5B4) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CREB3L1 Ready-To-Use IHC Kit. CREB3L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67617-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67617 targets CREB3L1,OASIS in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat
Immunogen CREB3L1,OASIS fusion protein Ag28042 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cAMP responsive element binding protein 3-like 1
Synonyms CREB3L1, OASIS
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC015781
Gene Symbol CREB3L1
Gene ID (NCBI) 90993
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96BA8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CREB3L1,OASIS antibody CL488-67617Download protocol
Standard Protocols
Click here to view our Standard Protocols