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CUL4B antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 61445,61446 61445

Synonyms

CUL4B, Cullin 4B, MRXSC, CUL-4B, SFM2, MRXHF2, Cullin-4B, MRXS15, histone h2a, histone h3, histone h4, dna damage, chromatin, h3, h4, ubiquitination, antibody, polyclonal, pAb, western blotting, wb, sample

验证数据展示

  • CUL4B (pAb) tested by Western blot. Detection of CUL4B by Western blot analysis. Nuclear extract of MCF-7 cells (20 ug) was probed with CUL4B (pAb) at a 1:500 dilution.

    CUL4B (pAb) tested by Western blot. Detection of CUL4B by Western blot analysis. Nuclear extract of MCF-7 cells (20 ug) was probed with CUL4B (pAb) at a 1:500 dilution.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human CUL4B.
Full Name CUL4B antibody (pAb)
Synonyms CUL4B, Cullin 4B, MRXSC, CUL-4B, SFM2, MRXHF2, Cullin-4B, MRXS15, histone h2a, histone h3, histone h4, dna damage, chromatin, h3, h4, ubiquitination, antibody, polyclonal, pAb, western blotting, wb, sample
Molecular weight 110 kDa
GenBank accession numberNP_003579
RRIDAB_2793640
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

CUL4B (Cullin 4B) is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, Histone H2A, Histone H3 and Histone H4 in response to radiation-induced DNA damage and may act as a transcriptional corepressor by ubiquitination of Histone H2AK119 and recruiting PRC2 complexes.