验证数据展示
经过测试的应用
| Positive WB detected in | HaCaT cells, SH-SY5Y cells, Calu-3 cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60123-1-Ig targets CXCL16 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CXCL16 fusion protein Ag4883 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-X-C motif) ligand 16 |
| 别名 | Small-inducible cytokine B16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, CXCL 16, C-X-C motif chemokine 16, C X C motif chemokine 16 |
| 计算分子量 | 273 aa, 30 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC017588 |
| 基因名称 | CXCL16 |
| Gene ID (NCBI) | 58191 |
| RRID | AB_2086188 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H2A7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CXCL16 is a recently discovered cytokine belonging to the CXC chemokine family, which is synthesised in plasmacytoid dendritic cell as a transmembrane molecule. It exists in a transmembrane and soluble form. The transmembrane form of CXCL16 functions as an adhesion molecule for CXCR6-expressing cells, whereas the soluble form of CXCL16 mediates infiltration of circulating cells into sites of injury. CXCL16, has been proposed as an important pathogenic mediator in inflammatory diseases, including rheumatoid arthritis, glomerulonephritis, or prostate cancer. CXCL16 has been implicated in some forms of renal disease such as lupus nephritis and antiglomerular basement membrane nephritis. CXCL16 also plays a pivotal role in the pathogenesis of angiotensin II-induced renal injury and fibrosis through regulation of macrophage and T cell infiltration and bone marrow-derived fibroblast accumulation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CXCL16 antibody 60123-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Activation of Piezo1 contributes to matrix stiffness-induced angiogenesis in hepatocellular carcinoma | ||
FASEB J Pathogenic Tconvs promote inflammatory macrophage polarization through GM-CSF and exacerbate abdominal aortic aneurysm formation. | ||
Mol Med Rep oxLDL promotes podocyte migration by regulating CXCL16, ADAM10 and ACTN4.
| ||
Cardiovasc Diabetol Klotho inhibits renal ox-LDL deposition via IGF-1R/RAC1/OLR1 signaling to ameliorate podocyte injury in diabetic kidney disease | ||
Chin J Nat Med Picroside II promotes HSC apoptosis and inhibits the cholestatic liver fibrosis in Mdr2-/- mice by polarizing M1 macrophages and balancing immune responses | ||
Cell Biochem Biophys Hinesol attenuates DSS-induced ulcerative colitis through the suppression of Src-mediated NF-κB and chemokine signaling pathway |
