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- KD/KO Validated
CYP1A1 Polyclonal antibody
CYP1A1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig and More (2)
Applications
FC, IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse cerebellum tissue, mouse lung tissue, HepG2 cells, HeLa cells, K-562 cells |
Positive IP detected in | mouse lung tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13241-1-AP targets CYP1A1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB,ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, pig |
Cited Reactivity | human, mouse, rat, zebrafish, pig |
Immunogen | CYP1A1 fusion protein Ag3990 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cytochrome P450, family 1, subfamily A, polypeptide 1 |
Synonyms | AHH, AHRR, CP11, CYP1, CYP1A1, CYPIA1, Cytochrome P450 1A1, Cytochrome P450 C, Cytochrome P450 form 6, Cytochrome P450 P1, P1 450, P450 C, P450DX |
Calculated Molecular Weight | 512 aa, 58 kDa |
Observed Molecular Weight | 50-58 kDa |
GenBank Accession Number | BC023019 |
Gene Symbol | CYP1A1 |
Gene ID (NCBI) | 1543 |
RRID | AB_2877928 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P04798 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
IHC protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
IF protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
IP protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
FC protocol for CYP1A1 antibody 13241-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Environ Int Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR) | ||
Free Radic Biol Med 5-Methoxyflavone ameliorates non-alcoholic fatty liver disease through targeting the cytochrome P450 1A1 | ||
Phytother Res Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity | ||
Environ Pollut Regioselective hydroxylation of carbendazim by mammalian cytochrome P450: A combined experimental and computational study. | ||
BMC Med NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma | ||
J Gastroenterol Ferroptosis in the colon epithelial cells as a therapeutic target for ulcerative colitis |