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验证数据展示

  • WB analysis of HEK-293 using 67419-1-Ig

    WB analysis of HEK-293 using 67419-1-Ig

    WB result of CYP2W1 antibody (67419-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP2W1 transfected HEK-293 cells.

  • WB analysis using 67419-1-Ig

    WB analysis using 67419-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67419-1-Ig (CYP2W1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67419-1-Ig

    IHC staining of human colon cancer using 67419-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67419-1-Ig (CYP2W1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HUVEC using 67419-1-Ig

    FC experiment of HUVEC using 67419-1-Ig

    1x10^6 HUVEC cells were intracellularly stained with 0.4 ug CYP2W1 Monoclonal antibody (67419-1-Ig, Clone:2B10H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 67419-1-Ig

    IF Staining of HepG2 using 67419-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP2W1 antibody (67419-1-Ig, Clone: 2B10H2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CYP2W1 Monoclonal antibody

CYP2W1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67419-1-Ig

产品说明书

CloneNo. 2B10H2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHUVEC cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67419-1-Ig targets CYP2W1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CYP2W1 fusion protein Ag29982 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cytochrome P450, family 2, subfamily W, polypeptide 1
别名 EC:1.14.14.-, Cytochrome P450 2W1, CYPIIW1, 2B10H2
计算分子量 54 kDa
观测分子量 45-54 kDa
GenBank蛋白编号BC025761
基因名称 CYP2W1
Gene ID (NCBI) 54905
RRIDAB_2882659
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TAV3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome P450s have been proposed to be of importance both in carcinogenesis, by activating precarcinogens, and as determinants of cancer chemotherapy, where they participate in activation or inactivation of anti-cancer drugs. CYP2W1 is exclusively expressed in transformed tissue in the adult human, mainly in colon tumors. It is a potential drug target in cancer therapy. CYP2W1 has a molecular weight of 52-54 kDa, and 30/45 kDa of potential isoforms.

实验方案

Product Specific Protocols
WB protocol for CYP2W1 antibody 67419-1-IgDownload protocol
IHC protocol for CYP2W1 antibody 67419-1-IgDownload protocol
IF protocol for CYP2W1 antibody 67419-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols