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CYR61 Monoclonal antibody

CYR61 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E11F11

Cat No : 67656-1-Ig

Print datasheet

Synonyms

CCN family member 1, CCN1, CYR61, GIG1, IBP 10, IGF binding protein 10, IGFBP 10, IGFBP10, Protein CYR61, Protein GIG1

验证数据展示

  • WB analysis using 67656-1-Ig

    WB analysis using 67656-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67656-1-Ig (CYR61 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67656-1-Ig

    IHC staining of human liver cancer using 67656-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYR61 antibody included
  • IHC staining of human liver cancer using 67656-1-Ig

    IHC staining of human liver cancer using 67656-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CYR61 antibody included
  • IF Staining of MCF-7 using 67656-1-Ig

    IF Staining of MCF-7 using 67656-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CYR61 antibody (67656-1-Ig, Clone: 2E11F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of A431 using 67656-1-Ig

    IF Staining of A431 using 67656-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CYR61 antibody (67656-1-Ig, Clone: 2E11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inU2OS cells, A431 cells, PC-3 cells, HeLa cells, MDA-MB-231 cells
Positive IHC detected inhuman liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy CYR61 Ready-To-Use IHC Kit. CYR61 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67656-1-Ig targets CYR61 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen CYR61 fusion protein Ag25129 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cysteine-rich, angiogenic inducer, 61
Synonyms CCN family member 1, CCN1, CYR61, GIG1, IBP 10, IGF binding protein 10, IGFBP 10, IGFBP10, Protein CYR61, Protein GIG1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC001271
Gene Symbol CYR61
Gene ID (NCBI) 3491
RRIDAB_2882854
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00622
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYR61 antibody 67656-1-IgDownload protocol
IHC protocol for CYR61 antibody 67656-1-IgDownload protocol
IF protocol for CYR61 antibody 67656-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Hum Cell

Long non-coding RNA CCAT2 drives the growth of laryngeal squamous cell carcinoma via regulating YAP activity.

Authors - Yanzi Zang
View Article
humanWB

Environ Toxicol

Inhibition of Ras protein activator like 2 produces antitumor effects in gastric cancer via the suppression of YAP1 activation

Authors - Yuan Hu
View Article