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Cardiac Troponin T Monoclonal antibody

Cardiac Troponin T Monoclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, rabbit, pig, chicken

Applications

IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4E2D8

Cat No : 68300-1-Ig

Print datasheet

Synonyms

Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle

验证数据展示

  • WB analysis of pig heart using 68300-1-Ig

    WB analysis of pig heart using 68300-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of rabbit heart using 68300-1-Ig

    WB analysis of rabbit heart using 68300-1-Ig

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat heart using 68300-1-Ig

    WB analysis of rat heart using 68300-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 68300-1-Ig

    WB analysis of mouse heart using 68300-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of chicken heart using 68300-1-Ig

    WB analysis of chicken heart using 68300-1-Ig

    chicken heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat heart using 68300-1-Ig

    IHC staining of rat heart using 68300-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68300-1-Ig (Cardiac Troponin T antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 68300-1-Ig

    IF Staining of mouse heart using 68300-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using cTnT antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of H9C2 using 68300-1-Ig

    IF Staining of H9C2 using 68300-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inrat heart tissue, pig heart tissue, rabbit heart tissue, mouse heart tissue, chicken heart tissue
Positive IHC detected inrat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, H9C2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

68300-1-Ig targets Cardiac Troponin T in WB, IF, IHC, ELISA applications and shows reactivity with mouse, rat, rabbit, pig, chicken samples.

Tested Applications IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity mouse, rat, rabbit, pig, chicken
Cited Reactivitymouse
Immunogen Cardiac Troponin T fusion protein Ag7716 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name troponin T type 2 (cardiac)
Synonyms Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle
Calculated Molecular Weight 36 kDa
Observed Molecular Weight34-40 kDa
GenBank Accession NumberBC002653
Gene Symbol TNNT2
Gene ID (NCBI) 7139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP45379
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.

实验方案

Product Specific Protocols
WB protocol for Cardiac Troponin T antibody 68300-1-IgDownload protocol
IHC protocol for Cardiac Troponin T antibody 68300-1-IgDownload protocol
IF protocol for Cardiac Troponin T antibody 68300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Clin Exp Hypertens

Exploration and validation of signature genes and immune associations in septic cardiomyopathy

Authors - Zhengbo Zhao
View Article